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At the time a medium is inoculated with spores, or launched and absorbed into the host substrate or medium, a network of stringy white branching filaments known as the mycelium will kind.

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Retain spore syringes from the refrigerator until able to use, particularly when you end up picking not to inoculate promptly, or when you have some spores left above from an inoculation.

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Syringe dimensions are generally 10cc (or 10mL). A median of 2-4cc of spore solution is normally important to reach proper colonization. It’s suggested to not exceed the typical injection level of two-4cc, in order to lessen surplus humidity within substrate containers, which could lead to irreversible mold development.

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Spore sellers will even ship a sterile needle attachment that is often affixed to a syringe to introduce or inoculate the spores directly in to the substrate. Even though these needles are shipped sterile, as with most mushroom cultivation techniques, usually re-sterilize them before use.